砷致小鼠脂质过氧化及锌、硒对砷的干预实验研究

Experimental study on intervene action of zinc, selenium on arsenic induced lipid peroxidation in mice

【目的】探讨砷对小鼠血清及肝脏脂质过氧化的影响及锌、硒对它的干预作用。【方法】采用亚慢性毒性实验方法,实验结束后分别测定小鼠血清、肝脏组织中脂质过氧化产物丙二醛(MDA)和谷胱甘肽S-转移酶(GST)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力,同时观察锌、硒干预后对染砷小鼠体内抗氧化水平的影响。【结果】随着砷染毒剂量的增加,小鼠血清及肝脏组织中MDA和GST含量显著增加,SOD、GSH-Px活性显著降低,且呈剂量-效应关系。锌、硒能抑制MDA和GST生成量,提高SOD、GSH-Px活性。【结论】砷可致机体脂质过氧化,锌、硒可拮抗砷的脂质过氧化作用。

To investigate the effect of arsenic on lipid peroxidation in mice liver and observe the intervention effect of zinc and selenium supplement [Methods] Subchronic toxicity experiment is applied. Then the mice organism and serum contents of lipid peroxides degradation product malondialdehyde （MDA）, the contents of glutathione S-transferase （GST）, the total superoxide dismutase （SOD） activities and glutathione peroxidase （GSH-Px） activities in serum and liver are determined individually. Meanwhile, study the influence of anti-oxidation activity in mice exposures to arsenic with zinc and selenium supplement. [Results] The results show that with the increases of exposure levels of arsenic, the contents of MDA and GST are significantly increased and the activities of SOD and GSH-Px are significantly decreased and show a dose-effect manner. Zinc and selenium can decrease the contents of MDA and GST and increase the activities of SOD and GSH-Px significantly. [Conclusion] Arsenic could induce lipid peroxidation in mice and zinc and selenium can inhibit the lipid peroxidation after arsenic exposure.