缩酮丙酮酸转移酶基因在野油菜黄单胞菌中的表达

EXPRESSION OF THE KETAL PYRUVATE TRANFERASE GENE IN XANTHOMONAS CAMPESTRIS NK01·S·1 STRAIN

将野油菜黄单胞菌（Ｘａｎｔｈｏｍｏｎａｓｃａｍｐｅｓｔｒｉｓ）ＮＲＲＬ－Ｂ－１４５９菌株的缩酮丙酮酸转移酶基因，在ｐＲＫ２０７３辅助质粒的协助下，经三亲本接合法，导入野油菜黄单胞菌ＮＫ０１小菌落的抗性突变株（ＮＫ０１·Ｓ·１）中，测定各接合子的产胶率、黄原胶粘度及丙酮酸含量，得到一系列优于原始菌株的接合子，其中四株的产胶率、丙酮酸含量及黄原胶粘度分别较出发菌株提高７．２８～１０．６０％、４０．２４～４１．４２％和１０．７９～２２．３０％．

The ketal pyruvate transferase gene from Xanthomonas campestris NRRL B 1459 were transferred in to Xanthomonas campestris NK01·S·1 by triparental conjugation. The exoconjugant dettrminations indicated that the yield rate, pyruvate content and viscosities of xanthan gum were increased by 7.28%￣10.60%, 37.28%￣41.42% and 10.79%￣22.30%, respectively.