血小板膜受体P2Y12基因多态性与原发性高血压血瘀证的相关性研究

Relationship between polymorphisms of P2Y12 receptor gene of platelet and essential hypertension with blood stasis syndrome （血瘀证） of traditional Chinese medicine

目的探讨血小板膜受体P2Y12基因G52T、-T744C多态性与原发性高血压（EH）血瘀证易感性的关系。方法选择福建省汉族人群EH血瘀证患者216例，EH非血瘀证患者220例，血压正常对照者193例。抽取受试者外周静脉血，提取DNA，采用聚合酶链反应-限制性内切酶片段长度多态性（PCR—RFLP）检测P2Y12基因G52T、-T744C多态性类型。结果3组间G52T的GG、GT和TT基因型分布频率差异无统计学意义（x2=2．041，P=0．728），-T744C的TT、TC和CC基因型分布频率差异有统计学意义（x2=10．500，P=0．033）；经进一步誓分割检验显示，EH血瘀证组TC／CC基因型分布频率（43．1％）高于EH非血瘀证组（30．9％）和健康对照组（33．2％），差异有统计学意义（X2=7．598，P=0．006）。多因素Logistic回归分析显示，以健康对照组为参照系，TC／CC基因型人群是患EH血瘀证的独立危险因素；调整相关混杂因素后，TC／CC基因型人群患EH血瘀证的相对危险度是1．518，95％可信区间（95％CI）为1．011～3．025，P=0．042。结论P2Y12基因TC／CC型可能是汉族人群EH血瘀证发病的易感危险因素之一。

Objective To explore the relationship between the G52T, -T744C polymorphisms of P2Y12 receptor gene of platelet and essential hypertension （EH） with blood stasis syndrome （血瘀证, BSS） of traditional Chinese medicine （TCM）. Methods All the patients were of Han nationality （汉族） in Fujian Province in China. Peripheral blood was collected for DNA examination. The G52T, -T744C genotype of P2Y12 gene in 216 patients of EH of BSS type （EH-BSS group）, 220 patients of EH of non-BSS type （EH-non-BSS group） and 193 healthy subjects （control group） was determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） assay. Results The constituent ratio of G52T GG, GT and TT genotype was not significantly different among the three groups （X2 = 2. 041, P = 0. 728） ; while the constituent ratio of -T744C TT, TC and CC genotype was significantly different among the three groups （X2= 10. 500, P= 0. 033）. Partitions of X2 method showed that the frequencies of -T744C TC/CC genotype in patients with EH-BSS （43.1%） were higher than those of EH-non-BSS group （30.9%） and control group （33.2%, x2=7. 598, P=0. 006）, and multinomial Logistic-regression indicated that -T744C TC/CC geno- type in a person was an independent risk factor for contracting EH-BSS with the reference category of control ; the odds ratio （OR） of -T744C TC/CC genotype for EH-BSS was 1. 518 （95% confidence interval： 1. 011 - 3.025, P = 0. 042） after adjusting for age, gender, fasting plasma glucose, creatinine, body mass index （BMI）, uric acid and blood fat. Conclusion T744C TC/CC genotype is possibly one of the risk factors susceptible to EH-BSS in the Chinese mass of Han nationality.