干酪乳杆菌LC2W细胞壁组分体外对巨噬细胞活性的影响

The Effect of Cell Wall Components of L.casei LC2W on Activity of Murine Macrophage Cells

探讨干酪乳杆菌LC2W细胞壁组分体外对小鼠巨噬细胞功能的影响。以培养液单纯培养小鼠巨噬细胞系RAW264.7细胞作为对照,研究干酪乳杆菌LC2W细胞壁主要组分磷壁酸和肽聚糖对RAW264.7细胞代谢水平、吞噬中性红能力及释放NO的影响。不同浓度磷壁酸和肽聚糖对小鼠巨噬细胞RAW264.7细胞代谢MTT能力、吞噬中性红能力有明显增强作用,并呈一定的剂量效应,在相同质量浓度时,两种细胞壁组分激活巨噬细胞能力无显著差异。同时诱导产生NO量也随着浓度增大而增加,当浓度达到50μg/mL时,磷壁酸诱导能力显现出较高的水平。干酪乳杆菌LC2W细胞壁主要组分磷壁酸和肽聚糖能激活小鼠巨噬细胞RAW264.7细胞,提高其代谢水平及吞噬能力,同时可诱导具有杀瘤作用的活性因子,并具有一定的剂量效应。

To investigate the effect of cell wall components of L.casei LC2W on the function of murine macrophage cells in vitro.The energy metabolism level,the phagocytic ability to neutral red of murine macrophage RAW264.7 cells and the content of NO derived from the cells were detected with different levels of teichoic acid（TA） or peptidoglycan（PG）,two predominant components of the cell wall of the cell L.casei LC2W,compared with those macrophage cells cultured without TA or PG.The energy metabolism level,the phagocytic activity were significantly hig her than those in control group,and were also in a dosage-dependent manner,no obvious difference existed in RAW264.7 cells challenged with Lactobacillus TA and those cells challenged with PG.The content of NO derived from murine macrophage RAW264.7 cells macrophages induced by different levels of TA or PG was enhanced and when it′s 50 μg/mL,the content of NO induced by TA is higer than that by PG.TA and PG of the cell wall of L.casei LC2W could activate macrophage RAW264.7 cells to secrete active factors with tumoricidal activity,and enhance its energy metabolism level,phagocytic activity to neutral red significantly in a dosage-dependent manner.