高效提取越橘成熟组织基因组DNA的方法

An Efficient Method for Genomic DNA Extraction from Mature Tissues of Blueberry(Vaccinium spp.)

为从越橘成熟组织中提取高质量DNA,对CTAB法进行改良,并与常规CTAB法及快速CTAB法的提取效果进行比较。结果表明：改良方法提取获得的DNA产量大、纯度高,DNA产量平均为192μg/g,OD260/OD280在1.75-1.85之间,所得DNA能被限制性内切酶完全消化,以其为模板进行SRAP-PCR扩增,能得到清晰、稳定的条带,证明改良方法适于越橘成熟组织DNA的提取。

To obtain high quality genomic DNA from mature tissues of blueberry（Vaccinium spp.）,a modified CTAB method was established.And then three different methods,the conventional CTAB method,rapid CTAB method and modified CTAB method,were compared.The results showed that the modified method could obtain the expecting DNA that reached the higher purity（OD260/OD280 is between 1.75 and 1.85） and higher yield（192 μg/g）.Furthermore,the DNA can be digested completely with restriction endonuclease,and the electrophoresis bands of SRAP-PCR were clear and stable.It is concluded that this modified CTAB method should be suitable for extraction of genomic DNA from mature tissues of blueberry（Vaccinium spp.）.