绿色木霉菌株Tv04-2固体发酵条件研究

Study on Fermentation Conditions of Antagonistic Trichoderma viride Tv042 Strain

以产孢量为测定指标,通过单因子和正交试验对人参根部病害拮抗绿色木霉菌株Tv04-2固体发酵条件进行了筛选,获得了以玉米粉、麦麸等廉价农产品及其副产物为碳、氮源的最佳固体发酵培养基,其配方为:在250 mL三角瓶中装入20 g固体基质,其中玉米粉∶麦麸按1∶1比例混合,FeSO4含量0.1 g,水7 mL,接种量4 mL;最佳发酵条件为:25,20和28℃下依次发酵2 d。利用优化培养基及发酵条件进行发酵试验,可以获得高达4×109cfu/g的产孢量,为人参根部病害高拮抗活性木霉菌剂的生产和应用奠定了基础。

Optimal solid-state fermentation conditions were studied according to the sporulation quantity of Trichoderma viride isolate Tv04-2 by simple factor and orthogonal experiments.The most optimal medium was formulated as following： 250 mL erlenmeyer flask contained 20 g of equal quality of maize powder and wheat bran mixture,0.1 g FeSO4,7 mL water and 4 mL inoculum.The fermentation process was at a fluctuant temperature of 25,20 and 28℃ for 2 days,respectively.Under this optimal medium and fermentation process,the spore concentration of 4×109 cfu/g can be obtained,and which accelerated the production and application of Trichoderma viride strain with high antagonistic activity of ginseng root diseases.