姜夏止咳汤的急性毒性及止咳祛痰药效研究

Investigation on the effect of JiangXiaZhikeTang

目的 研究姜夏止咳汤的急性毒性及主要药效学,以为该方的进一步开发提供实验数据.方法 采用最大给药量法测定急性毒性,氨水、二氧化硫引咳法观察止咳作用.气管酚红排泄法、毛细血管排痰法观察祛痰作用.采用二氧化硫慢性刺激诱导慢性支气管炎模型,观察止咳作用及抗氧化的作用.结果 姜夏止咳汤1次最大给药量为436 g原药材/kg体重,相当于成人日用量的968倍.可抑制氨水以及二氧化硫所致小鼠咳嗽反应.可促进小鼠气管酚红的排泄;并能促进大鼠毛细血管排痰.慢性支气管炎模型治疗后体重下降、咳嗽次数减少,肺组织匀浆SOD,GSH-Px活力升高,MDA含量下降,血清MPO含量降低;胸腺指数、脾指数均明显升高.结论 姜夏止咳汤安全有效,具有确切的止咳和祛痰作用,可对抗机体氧化损伤,增强机体抗氧化酶的活力,改善慢性支气管炎存在的氧化-抗氧化失衡,提高免疫功能.

Objective Investigate the acute toxicity and chief effects of JiangXiaZhiKeTang to provide its further development with experimental data. Methods Asminist the maximal dose of JiangXiaZhiKeTang to determine the acute toxicity of JiangXiaZhiKeTang on mice. Observe the antitussive effect of JiangXiaZhiKeTang on mice cough induced by ammonia and sulfur dioxide. Determine the expectorant effect of JiangXiaZhiKeTang by measuring the phenol red excreted in mice trachea or measuring the amount of phlegm siphoned by capillary. Build a chronic bronchitis animal model by sulfur dioxide, intrapulmonary superoxide dismutase （SOD）, glutathione peroxidase （ GSH-Px ）, malondialdehyde （ MDA ） and myeloperoxidase（ MPO ） in blood serum as indexes to evaluate JiangXiaZhiKeTang, as well as avoirdupois, the change in immune organs frame and tussive frequency. Results The primary maximal dose of JiangXiaZhiKeTang is 436 grams crude drug per kilogram with mouse, 968 times as many as adult daily dose. JiangXiaZhiKeTang can relieve a cough induced by ammonia and sulfur dioxide, promote the excrete of phenol red and phlegm. Compared to chronic bronchitis animal model group, superoxide dismutase （ SOD ）, glu- tathione peroxidase（GSH-Px ）are raised, prominently thymus index and spleen index, but cough frequency, malondialdehyde （MDA ） content , myeloperoxidase （ MPO ） content of treatment group are all reduced. Conclusions With exact antitussive and apophlegmatic effect, JiangXiaZhiKeTang is safe and effective. JiangXiaZhi KeTang can improve the unbalance between oxidation and anti-oxidation within chronic bronchitis and immunologic function by way of reinforce antioxidase activity.