红叶石楠离体培养与高效再生体系建立

Construction of the Regeneration System for in Vitro Culture of Photinia×frasery

以红叶石楠(Photinia×frasery)带芽茎段、叶片为外植体,对其启动培养、叶片愈伤诱导与分化、芽增殖、生根培养及试管苗驯化等技术进行了较为系统的研究,建立了红叶石楠离体培养的高效再生体系。结果表明,幼茎启动培养适宜培养基为MS+6-BA3.0mg/L+NAA0.3mg/L+GA0.5mg/L+蔗糖30g/L+琼脂7g/L,诱导率达63.3%;叶片愈伤诱导适宜的培养基为MS+6-BA0.5mg/L+2,4-D1.0mg/L+NAA1.0mg/L+蔗糖30g/L+琼脂7g/L,愈伤诱导率达86.0%;愈伤分化不定芽的适宜培养基为N6+6-BA1.5mg/L+IAA0.1mg/L+蔗糖30g/L+琼脂7g/L,诱导率达21.6%,增殖率为1.4倍;芽增殖培养适宜的培养基为MS+6-BA3.0mg/L+KT0.5mg/L+NAA0.3mg/L+GA0.7mg/L+蔗糖30g/L+琼脂7g/L,最高增殖率达6.1倍;生根培养方法为用25mg/LNAA溶液浸泡0.5～1.0h后接种于1/2MS培养基中,生根率达93.4%以上;试管苗驯化的适宜混合基质为泥炭∶珍珠岩=3∶1,成活率可达96.7%。

Photinia×frasery stem and leaves were used as the explants to study the effects of external hormone on the buds induction, differentiation, multiplication and roots formation.The results showed that in the medium of MS＋6-BA 3.0 mg/L＋ NAA 0.3 mg/L＋GA 0.5 mg/L＋ sucrose 30 g/L＋agar 7g/L, the percentage of induction was 63.3%; In the medium of MS＋6-BA 0.5 mg/L＋2,4-D 1.0 mg/L＋NAA 1.0 mg/L＋sucrose 30g/L＋agar 7g/L, the calli forming rate was 86.0%; In the medium of N6＋6-BA 1.5 mg/L＋IAA 0.1 mg/L＋sucrose 30g/L＋agar 7g/L, the percentage of induction was 21.6%, multiplication times 1.4; In the medium of MS＋6-BA 3.0 mg/L＋KT 0.5 mg/L＋NAA 0.3 mg/L＋GA 0.7 mg/L＋sucrose 30g/L＋agar 7g/L, the multiplication times were 6.1; Explants soaked for 0.5～1.0 h after the 25mg/L NAA solution to vaccinate in the 1/2MS medium, the radication rate was more than 93.4%, using stroma of peat： perlite is 3∶1 to acclimate the test-tube plantlets, the survival rate was 96.7%.