盐胁迫下外源NO对玉米幼苗叶片蔗糖代谢的影响

Effect of Exogenous Nitric Oxide on Sucrose Metabolism of Maize Seedlings Leaves under Salt Stress

以50mmol/LNaCl模拟盐胁迫,利用一氧化氮(NO)供体硝普钠(SNP)及NO的专一清除剂c-PTIO处理盐胁迫下的玉米幼苗,探讨外源NO对盐胁迫下玉米幼苗叶片脯氨酸含量和蔗糖含量的变化。结果表明,外源的NO处理促进脯氨酸、蔗糖和可溶性总糖大量的积累,促进蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)活性的提高,提高叶片的相对含水量,降低叶片的相对电导率,从而提高了玉米幼苗对盐胁迫的抗性,当叶片的NO受到NO清除剂清除时,加剧了盐胁迫对玉米幼苗的毒害。

To investigate the effect of exogenous nitric oxide （NO） on the variation of proline and sucrose in maize seedlings leaves under salt stress, 50 mmol/L NaCl was employed to simulate salt stress, sodium nitroprusside as nitric oxide （NO） donor and c-PTIO as the NO specific scavenger were used to treat the stressed maize seedlings.The results suggested that with the treatment of exogenous NO, in the seedlings leaves, the accumulation of proline, sucrose, and soluable total sugar were greatly promoted, the activities of SPS and SS were enhanced, while the relative water content was raised, while the relative electric conductivity was decreased, and the resistance to salt stress of maize was enhanced.But when NO scavenger was used, the salt stress was increased.