雷公藤多甙片对血栓闭塞性脉管炎大鼠红细胞免疫功能和血液流变性的影响

Effect of Glucosidorum Tripterygll Totorum to Experimental Thromboangiitis Obliterans in Rats on Erythrocyte Immune Function and Hemorrheology

目的:观察雷公藤多甙片( GTT)对血栓闭塞性脉管炎( TAO)大鼠红细胞免疫功能和血液流变性的影响。方法:采用TAO大鼠,分为对照组( N组)、模型组( M组)、GTT组、己酮可可碱组( JTKKJ组)、甲氨蝶呤组( JADL组)等5组进行对比研究。实验开始时、第6周末及第12周末分别测定下述指标: TAO大鼠肢体病变分级( LAC)、红细胞C3 b受体花环率( RBC-C3 bRR)、红细胞免疫复合物花环率( RBC-ICR)和血液流变学指标全血粘度,血浆粘度,红细胞聚集指数,红细胞变形指数。结果:治疗前, M组、GTT组、JADL组和JTKKJ组的LAC、RBC-C3 bRR、RBC-ICR和血液流变学指标与N组比较具有显著性差异(P<0 .05 )。治疗6周末和12周末,GTT组、JADL组和JTKKJ组的LAC与同组治疗前比较具有显著性差异(P<0 .05 ) , GTT组和JADL组的RBC-C3 bRR、RBC-ICR与同组治疗前比较具有显著性差异(P<0 .05),GTT组的LAC分别与JADL组和JTKKJ组组间比较有显著性差异(P<0.05),GTT组与JADL组的RBC-C3bRR及RBC-ICR分别和JTKKJ组组间比较有显著性差异(P<0.05)。治疗12周末,GTT组、JADL组和JTKKJ组的LAC与同组治疗6周末比较无显著性差异(P>0.05),GTT组和JADL组的RBC-C3bRR、RBC-ICR与同组治疗6周末比较无显著性差异(P>0.05),GTT组和JTKKJ组的血液流变学指标与同组治疗前比较具有显著性差异(P<0.05),并且两组分别和JADL组组间比较有显著性差异(P<0.05)。结论:GTT可能通过提高红细胞免疫吸附功能及改善血液流变性而有效治疗TAO。

Objective:To study the effect of glucosidorum tripterygll totorum on erythrocyte immune function and hemorrheology in therapy of the thromboangiitis obliterans(TAO).Method:The rats model was established by tobacoo sensitization,cold,injection of TAO immune abstract and disturbance of sex hormone for 8 weeks.All rats are randomly divided five groups:normal group(N group),model group(M group),glucosidorum tripterygll totorum group(GTT group),pentoxifylline group(JTKKJ group) and methotrexate group(JADL group).At the treatment beginning,the sixth weekend and the twelfth weekend,the items as follows were measured:limbs affection class (LAC),red blood cell C3b receptor rosette(RBC-C3bRR),red blood cell immune complex rosette(RBC-ICR),ratio of blood viscosity(RBV),ratio of plasma viscosity(RPV),red blood cell aggregation index(RAI) and erythrocyte deformation index(EDI).Results:Before the treatment,there was significant difference between N group and the four groups of M,GTT,JADL and JTKKJ in LAC,RBC-C3bRR,RBC-ICR and items of hemorrheology(P<0.05).At the sixth or twelfth weekend of treatment,LAC of three groups of GTT,JADL and JTKKJ were significant difference with that at treatment beginning(P<0.05).There is significant difference between GTT group and JADL group with JTKKJ group in RBC-C3bRR and RBC-ICR.LAC of GTT group was significant difference with that of JADL group and JTKKJ group.At the twelfth weekend of treat-ment,LAC of three groups of GTT,JADL and JTKKJ was no significant difference with that at the sixth weekend of treatment(P<0.05).RBC-C3bRR and RBC-ICR of two groups of GTT and JADL were no significant difference with that at the sixth weekend of treatment(P<0.05).Items of hemorrheology of two groups of GTT and JTKKJ were significant difference with that at the experiment beginning (P<0.05),and there was significant difference between GTT group and JTKKJ group with JADL group respectively.Conclusion:GTT has good clinical effect to TAO through changing items of hemorrheology and improving immune adsorption function of red blood cell.