Ptcor8表达与枳生理落叶及温度的关系

Ptcor8 expression related to defoliation of Poncirus trifoliate Raf. and temperature

将干径粗度相近的2年生枳实生容器苗60株从田间移入温室(20～25℃),以田间10株为对照.从2008年11月14日至2009年1月3日,每10d从温室随机搬出3株置于田间,研究枳叶片中Ptcor8的表达与枳生理落叶及环境温度的关系.结果表明,温室里的枳冬季不落叶;从温室搬至田间的枳,随着搬出温室时间的延后,其落叶期推迟;12月中旬后搬出温室的枳,即使经历冬季低温也不落叶.在落叶和未落叶的枳叶片中,均能检测到Ptcor8的表达,qPCR结果表明,Ptcor8的表达与枳的生理落叶无关,其表达量的明显增加与骤然降温和持续低温有关,与低温关系密切.

To study Ptcor8 expression related to the defoliation of Poncirus trifoliate Raf. and the temperature, 2-year-old seedlings of 60 potted Poncirus trifoliata Raf. with similar diameter, in the temperature-controlled chamber （20～25 ℃）, were transferred to the field every 10 d from the 14th November, 2008 to January of next year, meanwhile, another 10 seedlings were put in the field as the control. The Poncirus trifoliate Raf. seedlings in the temperature-controlled chamber and those transferred to the field after the 14th December were non-deciduous. However, those transferred to the field before the 14th December was deciduous, and their defoliating period delayed along with the time outside the temperature-controlled chamber. In the deciduous and the non-deciduous the expression of Ptcor8 gene was both detected. Furthermore, by qPCR, the more changes of temperature and the longer duration of low temperature, the higher expression of Ptcor8. Therefore, the expression of Ptcor8 gene may not be directly related to the physiological defoliation of Poncirus trifoliate Raf., but rather closely related to low temperature induction.