卡前列甲酯合并酪氨酰肼对小鼠的抗早孕作用

Effect of 15 Methyl PGF 2α Methyl Ester Combined with Tyrosine Hydrazide on Early Pregnancy in Mice

目的观察卡前列甲酯（ＰＧ０５）合并酪氨酰肼对小鼠抗早孕作用。方法小鼠于妊娠第７～９天给药，观察妊娠率；用放射免疫法测定血清孕酮水平。结果小鼠口服９０ｍｇ／ｋｇ酪氨酰肼（３ｄ）能显著增强皮下注射０．２ｍｇ／ｋｇＰＧ０５（２ｄ）的抗早孕作用，并协同ＰＧ０５降低孕小鼠血清孕酮的水平。３０μｇ／ｍｌ酪氨酰肼对大鼠黄体细胞３β－ＨＳＤ的活性有明显的抑制作用。口服１５０ｍｇ／ｋｇ酪氨酰肼（３ｄ）对假孕大鼠子宫孕激素受体数目无显著影响。结论酪氨酰肼抑制体内孕酮的生成可能是其协同ＰＧ０５抗早孕作用的主要机制。

Objective To observe the effect of 15 methyl PGF2α methyl ester (PG05) in combination with tyrosine hydrazide (TH) on early pregnancy in mice.Methods The pregnancy rate of mice treated with PG05 and TH was counted and serum progesterone level was measured by RIA.Results Administration of TH at 90 mg·kg·day -1 to mice on day 7～9 of gestation enhanced significantly the termination of early pregnancy induced by PG05 at 0 2 mg -1 ·kg -1 for 2 days. Serum progesterone level of mice treated with PG05 combined with TH was found to be markedly lower than that of animals given PG05 or TH alone. TH at 30 μg -1 ·ml -1 inhibited significantly the activity of 3β HSD of luteal cells in rat. However,PG05 at 10 μg -1 ·ml -1 had no influence upon inhibitory effect of 3β HSD activity induced by TH.TH at 150 mg -1 ·kg -1 for 3 days did not affect the progesterone receptor level of utering cytoplasma in pseudopregnant rats. Conclusions The inhibitory effect of TH on the production of progesterone may be the main mechanism to terminate early pregnancy when used synergically with PG05.