核转录因子FOXP3蛋白在人脑星形细胞肿瘤组织中的表达及临床意义

Clinical significance of immunohistochemically detecting forkhead box P3 expression in human astrocytic tumors

目的:检测星形细胞肿瘤组织中FOXP3蛋白的表达,探讨星形细胞肿瘤组织中FOXP3的表达与临床病理特征以及预后的关系。方法:应用免疫组织化学技术检测121例星形细胞肿瘤和5例脑膜瘤,5例正常脑组织中FOXP3的表达。分析FOXP3的表达与星形细胞肿瘤各临床病理参数及预后的关系。结果:FOXP3蛋白在人脑星形细胞肿瘤组织中有表达,在脑膜瘤和正常脑组织中均未见表达,FOXP3蛋白主要表达于间质淋巴细胞的细胞核。在星形细胞肿瘤中,FOXP3的表达在各级别星形细胞肿瘤中表达差异显著(P<0.05)。Kaplan-Meier生存分析提示FOXP3阳性组和阴性组生存时间差异显著(P<0.01),但COX多因素变量分析结果显示,FOXP3不是影响星形细胞肿瘤患者术后生存时间的独立预后因素(P>0.05)。结论:FOXP3在星形细胞肿瘤间质淋巴细胞中的表达与星形细胞肿瘤的恶性程度以及病人的年龄相关。FOXP3阳性的星形细胞肿瘤的预后较FOXP3阴性的星形细胞肿瘤差,但不能作为影响星形细胞肿瘤生存时间的独立预后因素。FOXP3可能成为星形细胞肿瘤免疫调节的新靶点。

AIM： To investigate the expression of forkhead box p3（FOXP3） protein in astrocytic tumors,and analyze its clinical significance.METHODS： We measured the incidence of FOXP3 in 121 astrocytomas,5 meningiomas,5 normal brain tissues using immunohistochemistry,analyzed the correlation of the presence of FOXP3 with histopathologic features and survival.RESULTS： FOXP3 exists in human brain astrocytic tumors,which was not found in meningioma and normal brain tissues.FOXP3 was expressed in interstitial lymphocytes mainly,and expressed in tumor cells rarely.The differences on FOXP3 expression between all grade levels were statistically significant（P0.05）.The Kaplan-Meier survival analysis showed that there was significant difference between the two survival curves,and the survival rate of the group with negative FOXP3 expression was higher than that of the positive group.Multivariate Cox proportional hazards regression analysis indicated that FOXP3 could not serve as an independent prognostic factor of astrocytic tumors survival time（P0.05）.CONCLUSION： FOXP3 immunoreactivity is significantly associated with the age and the malignancy of human astrocytic tumors.Astrocytic tumor patients with FOXP3 expression have poorer prognosis,while FOXP3 can not serve as an independent prognostic factor of astrocytic tumors survival time.FOXP3 may provide a new target for immunotherapy of astrocytic tumors.