基底膜基质胶携带人脂肪组织来源间充质干细胞移植改善心肌梗死大鼠心脏功能

Functional improvement of infarct heart by implantation of human adipose-derived mesenchymal stem cells delivered by matrigel

目的:应用基底膜基质胶(matrigel)携带人脂肪组织来源间充质干细胞(ADMSCs)在大鼠的心肌梗死部位注射,观察其对细胞存活的影响及改善心梗模型大鼠心脏功能的能力。方法:分离、培养、扩增人ADM-SCs,左冠状动脉前降支结扎法建立大鼠急性心肌梗死模型。将大鼠急性心肌梗死模型随机分为4组,对照组(PBS组)、matrigel组、PBS+ADMSCs组、matrigel+ADMSCs组,对移植4周后细胞的存活及心梗局部新生血管的密度进行测量,并利用心脏超声检测大鼠心脏功能。结果:与其它各组相比,matrigel+ADMSCs组的细胞4周存活细胞数量以及心肌梗死区域新生血管密度均显著提高,心脏超声结果也表明该组大鼠的心脏功能改善最为显著。结论:基底膜基质胶能够提高人脂肪组织来源间充质干细胞在大鼠心肌梗死部位的存活,有助于提高心肌梗死部位微血管生成并改善心肌梗死大鼠的心脏功能。

AIM： To investigate the implantation of matrigel carrying human adipose-derived mesenchymal stem cells to enhance the cell survival and the improvement of the ventricular functions in infarct heart.METHODS： Human adipose-derived mesenchymal stem cells（ADMSCs） were isolated and cultured from adult adipose tissue.SD rats with one-week-old myocardial infarction were randomly received the following 4 treatments： injection of PBS,matrigel,PBS＋ADMSCs or matrigel＋ADMSCs,respectively.Labeled ADMSCs either in matrigel or in saline were injected into the border area of ischemia.The controls received the injection of matrigel or saline only.Four weeks after injection,the heart functions were determined by echocardiography.The densities of the micro-vessels within the infarct area were also measured.RESULTS： Four weeks after implantation of ADMSCs,the cell graft size,the heart functions and the micro-vessel densities within the infarct area improved in matrigel＋ADMSCs group as compared to other groups.CONCLUSION： The co-injection of ADMSCs with matrigel enhances the graft survival,increases the density of the micro-vessels in the myocardium and improves the cardiac functions.