光合放氧复合物锰簇光组装

Photoassembly of Manganese Cluster of Photosynthetic Oxygen-Evolving Complex

在光系统Ⅱ水氧化过程中,由锰簇及其附近一个具氧化还原活性的酪氨酸YZ组成的光合放氧复合物通过4个连续的氧化还原反应将水裂解为质子和氧气。在光系统Ⅱ的功能性组装过程中,光合放氧复合物锰簇是通过一个被称作光组装的过程形成的。结合当前的研究进展,本文详尽地介绍了光合放氧复合物锰簇光组装的动力学模型、与锰簇形成相关的蛋白及锰簇的外围配体、锰与去锰光系统Ⅱ的结合特性、其他辅助因子对锰簇光组装的影响及锰簇光组装的机理。此外,结合我们的研究结果,对人工合成的锰化合物与去锰光系统Ⅱ放氧复合物的光组装进行了综述和讨论。本文最后介绍了目前光合放氧复合物锰簇结构及功能研究中存在的一些问题,并对光合放氧复合物锰簇结构及功能研究的应用前景进行了展望。

In the process of water oxidation in photosystem Ⅱ（PS Ⅱ）,water is split into four protons and O2 via a series of four redox steps in a catalytic site called oxygen-evolving complex（OEC）,which consists of a manganese cluster and a redox-active tyrosine residue,YZ.During the assembly of PS Ⅱ to its functional state,the manganese cluster of photosynthetic OEC is formed by a process called photoassembly.In this paper,based on the current research progresses,a complete introduction on the photoassembly of the manganese cluster of photosynthetic OEC is presented as follow：（1） Kinetic model for photoassembly of the manganese cluster complex OEC;（2） Proteins and external ligands related to the formation of the manganese cluster in photoassembly;（3） The binding properties of manganese in the apo-OEC-PS Ⅱ;（4） Role of cofactors in photoassembly;（5） Mechanism for the photoassembly of the manganese cluster of photosynthetic OEC.In addition,the photoassembly of the manganese cluster by using synthetic manganese complex and apo-OEC-PS Ⅱ is reviewed and discussed on the bases of our research results.In the end of this paper,some open questions from the present research on the structure and function of the manganese cluster of photosynthetic OEC are pointed out and the future application from this research is introduced.