响应面法优化复合淀粉微球制备工艺研究

Study on optimization of preparation technology of composite starch microspheres by response surface methodology

以可溶性淀粉为主要原料,添加一定量β–环糊精,采用反相悬浮聚合法制备复合淀粉微球。为优化其制备工艺,在单因素试验基础上选取淀粉乳浓度、淀粉与β–环糊精质量配比、油水体积比和交联剂用量为影响因子,应用BBD进行4因素3水平试验设计,以淀粉微球对亚甲基蓝吸附量为响应值进行响应面分析,模拟得到二次多项式回归方程预测模型,并得到复合淀粉微球制备最优工艺条件为:淀粉乳浓度15.09%、淀粉与β–环糊精质量配比2.62∶1、油水体积比3.84∶1和交联剂用量6.13 ml;在此条件下,验证得到淀粉微球对亚甲基蓝吸附量为0.5337 mg/g,与预测值0.5659 mg/g相对误差为5.69%,说明应用响应面法所得复合淀粉微球制备工艺条件是可行的。

The composite starch microspheres were prepared by inverse–suspension polymerization with soluble starch as the main material at the same time adding a certain amount β–cyclodextrin （β–CD）.Based on the single–factor test,four independent variables including starch concentration、starch β–CD weight ratio、oil–water volume ratio and the amount of the cross–linking agent were selected as impact factor to optimize the preparation process.The experiments were arranged according to BBD（Box–Behnken Design）.RSA（Response Surface Analysis）method was applied to determine the effect of BBD and simulate the predicted model of the quadratic polynomial regression equation.The optimum conditions was obtained as follows：starch concentration 15.09%、starch β–CD weight ratio 2.62∶1、oil–water volume ratio 3.84∶1 and the amount of the cross–linking agent 6.13 ml.Under this condition,the experiment data is 0.5337 mg/g,with the relative error 5.69% compared with the predicted value,indicating this preparation process condition derived from RSA is feasible.