病毒性心肌炎柯萨奇B组病毒中和抗体的检测

Detection of specific anti-coxsackievirus B neutralizing antibodies in viral myocarditis

探讨抗柯萨奇B病毒（CVB）中和抗体在正常小儿的水平和诊断病毒性心肌炎中的作用。方法采用微量中和试验方法测定病毒性心肌炎患儿和正常小儿血清中抗CVB中和抗体水平，同时用ELISA方法测定心肌炎患儿CVB特异性IgM。结果正常小儿中和抗体检出率为77．27％（51／66），心肌炎组为95．28％（101／106）；心肌炎组中和抗体异常率73．58％（78／106），中和抗体异常儿中CVB－IgM阳性率为28．38％（21／74）。两组CVB型别分布均以B3感染为最多。结论病毒性心肌炎中以CVB3感染最为多见，因正常小儿CVB感染率很高，只有通过双份血清抗体效价4倍上升或下降才能证明近期存在感染。

To study the level of anti-coxsackievirus B (CVB) neutralizing antibody in normal children and its diagnostic effect of viral myocarditis.Methods: With the microtechnique of neutralization test. AntiCVB neutralizing antibodies in 106 cases of viral myocarditis and in 66 cases normal children were detected. At the same time, specific CVB IgM was detected with the ELLISA in viral myocarditis.Results: The detected rates of CVB neutralizing antibody in viral myocarditis and normal group were 95. 28% (101/106) and 81. 80% (54/66) respectively. Abnormal neutralizing antibodies in myocarditis group was 73. 58% (78/106). The detected rate of CVB specific IgM positive was 28. 38% in abnormal neutralizing antibody group.Conclusion: This study suggests that CVB3 is the major etiologic in viral myocarditis. Because the infection of CVB in normal children is very high. only the titer of antibodly in double serum is more than 4 times higher or lower the recent infection is proved present.Keyward: coxsackievirus; viral myocarditis; neutralizing antibody