多种乳腺癌相关基因mRNA联合检测在乳腺癌诊断中的应用

Clinical application of combined detecting for severel gene mRNA levels in diagnosis of breast cancer

目的:荧光定量聚合酶链反应(FQ-PCR)法检测细胞角蛋白19(CK19)、C-erbB2、核内原癌基因(c-myc)和细胞周期蛋白D1(ccnd1)mRNA水平,探讨联合检测在乳腺癌诊断和治疗监测中的应用。方法:建立FQ-PCR法,并以β2-微球蛋白为内对照测定15名健康女性体检者、30例良性乳腺疾病患者和81例乳腺癌患者外周血中四种基因的mRNA水平。结果:CK19、C-erbB2、c-myc和ccnd1的mRNA水平在正常对照组和良性乳腺疾病组间差异无显著性意义(P>0.05),乳腺癌组均高于前两组(P<0.05),β2-微球蛋白在三组间差异无显著性意义(P>0.05)。四种mRNA联合检测的灵敏度高于单个mRNA的检测。结论:FQ-PCR技术是灵敏度和特异性较高的快速定量检测CK19、C-erbb2、c-myc和ccnd1的mRNA方法,四者联合检测可提高对乳腺癌诊断的灵敏度。

Objective： To evaluate the clinical application of fluorescent quantification polymerase chain reaction （FQ-PCR） for combined detecting CK19 and C-erbB2 and c-myc and ccnd1 mRNA levels in breast cancer. Methods： Using β2-microprotein mRNA as internal control, four mRNA in peripheral blood samples from 15 healthy women, 30 patients with benign breast disease and 81 patients with breast carcinoma were detected by FQ-PCR. Results： No significant difference in four mRNA levels waw found between normal controls and benign breast disease group（P0.05）, four mRNA levels in breast cancer group were higher than those in the other groups（P0.05）. No difference was found in β2-microglobin among three groups（P0.05）. The sensitivity of four mRNA levels by combined detection was higher than that of single gene detection. Conclusion： FQ-PCR method is better to detect CK19, C-erbB2, c-myc and ccnd1 mRNA levels for its high sensitivity. The sensitivity of diagnosis for breast cancer can be improved by combined detection.