慢性髓系白血病中prame基因转录本的定量研究

Quantification of Prame Gene Transcript in Chronic Myeloid Leukemia

本研究旨在分析慢性髓系白血病(CML)患者中黑色素瘤优先表达抗原(prame)的基因转录本表达状况及其临床意义。用实时定量PCR(RQ-PCR)方法对30例CML患者和15例缺铁性贫血(IDA)患者骨髓单个核细胞中prame基因转录本水平进行检测。结果表明:15例缺铁性贫血患者骨髓单个核细胞(BMMNC)中prame转录本水平为0%-1.46%(中位0.19%),30例CML患者中BMMNC中prame基因转录本水平为0%-772.25%(中位8.28%),二组之间具有显著性差异(p<0.001)。CML患者中prame基因转录本含量与bcr-abl融合基因转录本水平显著相关(r=0.708,p<0.001);6例CML加速期(AP)和急变期(BC)患者中prame基因转录本水平明显高于24例慢性期(CP)患者(p=0.007)。动态检测2例CML患者在不同病程中prame基因转录本水平变化显示,AP及BC时prame基因转录本水平增高,经治疗后恢复至CP时prame基因转录本水平降低,与bcr-abl转录本变化趋势一致。结论:CML患者中prame基因转录本表达水平增高,且与病情发展相关,可用于病情监测。

This study was purposed to analyze the expression level of preferentially expressed antigen of melanoma （prame） transcript in the patients with chronic myeloid leukemia （CML） and explore its clinical significance. Real-time quantitative PCR （RQ-PCR） assay was used to detect the level of prame gene transcript in the bone marrow samples from 30 patients with CML and 15 patients with iron deficiency anemia （IDA）. The results showed that CML patients had significantly higher prame mRNA level （0% - 772.25%, median 8.28% ） than IDA cases （0% - 1.46%, median 0.19% ） （p 〈0. 001 ）. The level of prame gene transcript was significantly correlated with that of bcr-abl fusion gene transcript （r = 0, 708, p 〈 0.001 ） in CML patients. Furthermore, 6 patients in blastic crisis （BC） and accelerated phase （AP） had significantly higher prame gene transcript than that of 24 cases in chronic phase （CP） （p = 0. 007 ）. In 2 CML patients with sequential samples, prame gene transcript increased in AP and BC, compared with in CP, and was consistent with the altering tendency of bcr-abl transcript. It is concluded that the level of prame gene transcript increases in CML which associates with the progression of the disease, prame gene transcript level can be used for monitoring the disease state.