核黄素光化学法对血浆中革兰阳性和阴性指示菌的减除作用和对血小板P选择蛋白表达的影响

Effects of Riboflavin Combined with Potosensitization on Reduction of Gram-positive and Gram-negative indicating Germs in Plasma and P-selectin Expression of Apheresis Platelet Concentrates

本研究验证核黄素(维生素B2)光化学减除血浆中指示菌的实际效果和对机采浓缩血小板的活化影响。以大肠杆菌为革兰阴性和以金黄色葡萄球菌为革兰阳性指示病原体,观察核黄素结合紫外线照射的协同效应和灭菌效果;采用流式细胞术评估灭活处理后的机采浓缩血小板的活化状态。结果表明:50μmol/L核黄素结合6.2J/ml剂量紫外线辐照,T/E比值分别为1.44和1.58,降低大肠杆菌和金色葡萄球菌3.87和3.82Log数。血小板经病原体灭活处理后于22℃保存0天和5天时CD62p的表达率分别为4.92%和36.18%,与对照3.94%和32.03%相比表达率略有增加(p<0.05)。结论:核黄素与紫外线照射协同效应可以减除大肠杆菌和金黄葡萄球菌指示菌,对血小板无明显影响,液体血小板部分活化状态表现为贮存损伤,此损伤程度在可接受的范围之内。

This study was purposed to confirm the practical efficacy of reducing indicating germs suspended in plasma by riboflavin and photosensitized inactivation and to evaluate its influence on activation of apheresis platelet concentrates. The synergistic effects of riboflavin combined with ultraviolet irradiation on inactivation of germs were investigated by using Escherichia Coli（ E. coli） and Staphylococcus Aureus （ S. aureus） as Gram- and Gram^＋ indicating germs, respectively. The activation status of apheresis-platelet concentrates treated with riboflavin combined with ultraviolet irradiation was detected by flow cytometry. The results showed that when 50 μmol/L of riboflavin was combined with 6.2 J/ml of ultraviolet irradiation, the T/E ratios reached 1.42 for E. coli and 1.68 for S. Aureus, and reduction of E. Coli and S. Aureus were 3.87 Logs and 3.82 Logs respectively ; the CD62p expression level on germ-inactivated platelets stored at 22℃ for 0 and 5 days were 4.92% and 36.18% respectively, which slightly increased as compared with controls （3.94% and 32.03） % （p 〈 0. 05）. It is concluded that combination of riboflavin with ultraviolet irradiation displays well synergistic effects which can reduce E. Coli and S. Aureus counts, but no significantly influence on plaletess. The partial activation of liquid platelets mainly presents metabolism damage during storage, which is found at an acceptable level.