HPLC法测定金参润喉合剂中绿原酸的含量

Determination of chlorogenic acid in Jinshen Runhou Heji by HPLC

目的 :建立金参润喉合剂中绿原酸含量测定方法。方法 :HPLC法 ,以NOVA -PAKC18( 4.6× 2 0 0mm)为色谱柱 ,流动相为 0 .5%冰醋酸溶液 -甲醇 ( 75∶2 5) ,检测波长为 3 2 8nm。结果 :平均回收率为 1 0 2 .9% ,相对标准偏差为2 .7% (n =5)。结论 :与卫生部药品标准 (试行 )中的薄层扫描法相比 ,本法杂质分离更加有效。

OBJECTIVE:To develop the method for determination of chlorogenic acid in Jinshen Runhou Heji.METHODS:Using HPLC,the column was NOVA-PAK C 18 (4.6×200mm),the mobile phase consisted 0.5% ice acetic acid solution-methanol(75∶25),the detective wavelength was 328nm.RESULTS:The average recovery was 102.9%(RSD=2.7%,n=5).CONCLUSION:Compared the TLCS method in CP,this method is better in the separation of impurity and more accurate.