应用大载体-生物反应罐系统生产抗人B型红细胞单克隆抗体

PRODUCTION OF MONOCLONAL ANTI-HUMAN RED BLOOD CELL B ANTIBODY THROUGH MACROCARRIER-BIOREACTOR

应用体外细胞大量繁殖系统生产抗人B型红细胞单克隆抗体,结合细胞生长和分泌抗体的特性,设置离线和在线参数等,对细胞增殖状态和生产抗体进行连续控制。一次运转52天,共收获含抗体培液23.25L,血凝效价1:64～1:512,为批量生产以单克隆抗体作为人血型定型试剂提供了条件。

Hybridoma cells (SRBC-CI) secreting anti-B antibody were cultured in macroca-rrier-bioreactor (Bellco) system. Optimal conditions or setpoints for the growth of SRBC-CI cells and antibody production were initially determined by conventional cell culture methods. Over the course of 52-day running, culture media were harvested periodically and the cumulative amount was 23.3 liters. The HA titer ranged from 1:512 (8 liters) to 1:64 (6.4 liters). The product was tested extensively and found to be as good as or even better than human polyclonal ABO reagents.