环氧和酶-2、基质金属酶-2、9在子宫内膜息肉中表达和相关性研究

The Expression of cyclooxygenase-2,matrix metalloproteinase-2,matrix metalloproteinase-9 in endometrial polyps

目的检测环氧和酶-2(COX-2)、基质金属酶-2、9(MMP-2、9)在绝经前、后子宫内膜息肉及绝经后正常子宫内膜中表达,并对三者的表达率作相关性分析。方法收集本院自2006年1月至2009年6月间经宫腔镜探查并取活组织检查,经病理诊断为子宫内膜息肉的完整病史资料按绝经与否分两组,各取30例。选取自正常绝经后妇女因子宫脱垂或卵巢囊肿行全子宫切除标本中正常子宫内膜共30例为对照组,使用GnRHR免疫组化法检测3组中COX-2、MMP-2、MMP-9表达。结果子宫内膜息肉中,绝经前组的上皮和间质COX-2阳性和强阳性表达均高于绝经后组,差异具有统计学意义(P<0.05);绝经前组和绝经后组的上皮中MMP-2、MMP-9的阳性和强阳性表达率相近,差异无统计学意义(P>0.05),但间质中绝经后组两者的阳性和强阳性表达高于绝经前组,差异具有统计学意义(P<0.05)。绝经后正常子宫内膜存在COX-2、MMP-2、MMP-9表达。绝经后内膜息肉与绝经后正常内膜比较,息肉组中三个蛋白酶表达显著高于内膜组,差异具有统计学意义(P<0.05)。结论 COX-2、MMP-2、MMP-9在绝经前、后子宫内膜均有表达,提示此3种蛋白酶可能对子宫内膜息肉的形成有一定的作用。

Objective To investigate and analyse the expression of cyclooxygenase-2（COX-2）,matrix metalloproteinase-2 and-9（MMP-2 and MMP-9） in endometrial polyps from premenopausal and postmenopausal women.Methods choose each 30 premenopausal and postmenopausal endometrium polyps diagnosed by pathology after hysteroscopic biopsy in gynecology department of our hospital during Jan,2006 – Jun,2009.Choose postmenopausal normal endometrium as contral group.Immunoahistochamical analysis for COX-2,MMP-2 and MMP-9 were performed on formalin fixed,paraffin-embedded tissue using GnRHR technique.Rusults In both epothellial cells and stroma of premenopausal polys the expression of COX-2 was significantly higer compared to postmenopausal polys.（P〈0.05）.There was no other differences in the expression of MMP-2 and MMP-9 in the epothellial cells of premenopausal and postmenopausal polys（P〉0.05）.Stromal expressions of MMP-2 and MMP-9 in premenopausal polys were significantly higher than in postmenopausal.Conslusion Both polys of premenopause and postmenopause express COX-2,MMP-2,MMP-9 in epothium and stroma.Immunohistochemical expression of these markers mey be in？enced by the ststus of menopause.This result may suggest that these proteins play some roles in the formation of endometrial polyps.