大鼠次声刺激后脑组织谷氨酸的变化

Changes of glutamate in brain of rats exposed to infrasound

目的：观察大鼠暴露于次声环境后脑组织中谷氨酸（Ｇｌｕ）的变化．方法：把大鼠置于不同的次声环境中暴露２ｈ，观察８Ｈｚ／９０ｄＢ暴露组，８Ｈｚ／１２０ｄＢ暴露组，１６Ｈｚ／９０ｄＢ暴露组，１６Ｈｚ／１２０ｄＢ暴露组及正常对照组大鼠脑组织海马区Ｇｌｕ的变化规律．结果：与对照组相比，８Ｈｚ／１２０ｄＢ暴露组和１６Ｈｚ／１２０ｄＢ暴露组脑组织海马区谷氨酸含量明显增加（Ｐ＜０．０１）；１６Ｈｚ／１２０ｄＢ暴露组脑组织海马区谷氨酸含量较８Ｈｚ／１２０ｄＢ暴露组明显增加（Ｐ＜０．０１）．结论：次声对脑组织的作用效应与其频率及声压级等参数有关，其中关键因素是声压级．Ｇｌｕ的兴奋性毒性可能参与次声对脑组织的损害．

AIM: To investigate the changes of glutamate (Glu) in brain of rats exposed to infrasound. METHODS: Rat model was established by putting the rats in the environment of infrasound for two hours. The rats were divided into five groups: normal control, 8 Hz/90 dB, 8 Hz/120 dB, 16 Hz/90 dB and 16 Hz/120 dB. RESULTS: Compared with the control group, the content of Glu in cerebral hippocampus was higher in 8 Hz/120 dB group and 16 Hz/120 group(P<0.01);The elevation of Glu content in 16 Hz/120 dB group was more significant than that in 8 Hz/120 dB group (P<0.01). HZCONCLUSION: The infrasound effect on the brain is related to the frequency and sound grade, and the latter is the key factor. The exciting toxicity of Glu may play a more important role in the injury of brain by infrasound.