冠心病患者LDL亚组分分析初步报告

Analysis of low density lipoprotein subfractions in coronary heart disease

目的研究冠心病患者ＬＤＬ亚组分与正常人的差异。方法用非变性聚丙烯胺梯度凝胶电泳法分离ＬＤＬ后，扫描、计算机积分法分析３２例冠心病患者及３２例正常对照者的ＬＤＬ亚组分图谱。结果（１）冠心病组小密ＬＤＬ占总ＬＤＬ的（３８．０±１５．１）％，对照组占（１６．５±９．２）％，差异有显著性（Ｐ＜００１）。（２）小密ＬＤＬ与甘油三酯水平呈正相关，ｒ＝０．５４，Ｐ＜００５。结论（１）冠心病患者血浆小密ＬＤＬ水平增高，与冠心病发病可能有一定关系。（２）小密ＬＤＬ水平与甘油三酯水平正相关。

Objective To compare LDL subfractions in coronary artery disease (CAD) patients and normal individuals. Methods LDL subfractions in 32 CAD patients and 28 normal individuals were separated by 2% 16% non denaturing polyacrylamide gel electrophoresis. The LDL subfraction distributions were analyzed by the measurement of the area under the curve of the scanned gel. Results (1) Comparing with the normal individuals, the small dense LDL subfractions increased significantly in CAD patients (38±15.1% versus 16.5±9.2% of total LDL, P <0.01). (2) The levels of small dense LDL were significantly postively correlated with plasma triglyceride level. Conclusion The increase in small dense LDL fraction may be a risk factor of CAD.