血小板受体糖蛋白Ⅲa基因与心肌梗塞关系的研究

The relationship between platelet receptor glycoprotein IIIa gene and myocardial infarction in Chinese

目的探讨血小板受体糖蛋白（ＧＰＩＩａ）基因变异与心肌梗塞（ＭＩ）发病的关系。方法运用变性梯度凝胶电泳（ＤＧＧＥ）技术对４０例ＭＩ患者和相应数量健康人作对照行ＧＰＩＩａ基因全部外显子筛查。同时，以ＲＦＬＰ和ＡＳＯ技术检测８２例ＭＩ和６８例健康人对照的ＧＰＩＩａ基因ＰｌＡ多态性，并与５０例美国健康白种人比较。结果发现ＧＰＩＩａ基因外显子５中存在核苷酸静止突变；１５０例中国人的ＧＰＩＩａＰＩＡ多态基因型均为ＰｌＡ１／ＰｌＡ１，与美国白种人比较，差异有显著性。结论表明中国人群的血小板受体ＧＰＩＩａ基因与ＭＩ发病无相关性。

Objective To investigate the relationship between myocardial infarction and platelet glycoprotein receptor GPIIIa gene. Methods All exons of the GPIIIa gene were screened by denaturing gradient gel electrophoresis (DGGE) in 40 MI cases and number matched controls. Furthermore, P1A1/P1A2 polymorphism of GPIIIa gene was checked in 82 MI cases and 68 controls by RFLP and ASO, with the results being compared with 50 American Caucasian. Results A silent mutation existed in exon 5. The genotype of GPIIIa polymorphism was exclusively P1A1/P1A1 in Chinese, which differed significantly from that in American Caucasian. Conclusion Platelet receptor GPIIIa gene is not related to MI morbidity in Chinese.