丹参合剂抗脑缺氧作用的研究

EFFECTS OF COMPOUND RADIX SALVIA MILTIORRHIZA EXTRACTS ZANAINST ANOXIC BRAIN INJURY

目的：通过整体动物实验和离体海马脑片实验，研究丹参合剂的抗脑缺氧作用。方法：观察密闭三角烧瓶中小白鼠缺氧存活时间；观察大鼠离体海马脑片缺氧过程中缺氧损伤电位（ＨＩＰ）的出现率及缺氧后诱发顺向群峰电位（ＯＰＳ）的恢复率和恢复程度。结果：小白鼠缺氧存活时间（ｍｉｎ），用药组（３３．１６±６．８７）较正常对照组（２１．１±３．９７）显著延长（Ｐ＜０．０１）；用药组ＯＰＳ恢复率７１．４％、恢复程度９２．４±５３．２％、ＨＩＰ发生率１４．３％，对照组ＯＰＳ恢复率２０％、恢复程度３０．２±３６．６％、ＨＩＰ发生率８０％，组间３项指标比较，差异均有显著性（Ｐ＜０．０５）。结论：实验用丹参合剂有明显的抗脑缺氧作用，其作用机制可能与丹参降低神经元的兴奋性，减少能耗，从而保护脑细胞有关。

Objective: To study the protective effects of compound radix salvia miltiorrhiza (RSM) extracts against hypoxic brain injury. Method:BZMice were tightly closed in 150ml glass, survival time was then observed. During anoxia and after reoxygenation, changes of orthodromic population spikes (OPS) in area CA1 of rat hippocampal slice were investigated. Results:BZWhile giving compound RSM extracts, survival time of mice was significantly longer than that of control group. OPS was inhibited when the slices was exposed to compound RSM extracts. Recovery of OPS after reoxygenation in slices exposed to compound RSM extracts was significantly higher than that of untreated slices. Hypoxic injury potentials in compound RSM extracts treated slices occurred less than that in untreated slices. Conclusion: Compound RSM extracts could protect brain from anoxic injury. The mechanism may be that salvia miltiorrhiza reduces neuron's excitability and energy consumption, and thereby protect neurons.