IFN-α对人体液免疫功能调节作用的实验研究

THE STUDY ON THE IMMUNE REGULATORY FUNCTION OF INTERFERON- TO THE HUMORAL IMMUNITY OF HUMAN

目的：以狂犬病疫苗为抗原进行人体免疫实验，了解低剂量干扰素－α对人体的体液免疫功能的影响，并探讨其影响机制。方法：试验组：将疫苗于０、７、１４天时分别接种５．０、５．０、２．５ＩＵ，并在０天同时注射２０万单位的人白细胞干扰素（ＩＦＮ－α）；对照Ⅰ组：除不注射ＩＦＮ－α外，同试验组；对照Ⅱ组：仅按常规方法接种狂犬病疫苗。结果：抗体ＧＭＴ在７、１４天检测时，试验组均高于对照Ⅰ、Ⅱ组，尤较对照Ⅱ组有显著性；抗体阳转率在第７天时即显著高于对照Ⅰ、Ⅱ组；而达到保护水平的达标率在第７天时，试验组为１０％，对照Ⅰ、Ⅱ组均为０。结论：低剂量２０万单位的ＩＦＮ－α可使人体接受狂犬病疫苗免疫后的抗体产生速度加快、产量增高，即可有效增强人的体液免疫功能。

Objective :Vaccinating the human with the rabies vaccine to investigate the effects of the lower dose IFN- to the humoral immunity of human,and to discuss the affecting mechanism. Methods :Test group:Injecting 5.0,5.0,2.5 IU rabies vaccine on the 0, 7th,14th day respectively,and injecting 0.2 million IU IFN- simltaneously on the 0 day. Control group 1: Operating as the test group,but not injecting IFN-.Control group 2:Only injecting rabies vaccine with the routine schedule recommended by WHO. Results :When examined on the 7th and 14th days,the antibody's GMT ,positive rate and the rate of defensible titre of the test group were higher than those of the control group 1. Furthermore,they were significantly higher than those of the control group 2,but those of the control group 1 were not significantly higher. Conclusion :Lower dose-0.2 million IU -IFN- can enhance the ability of the immune system of human body to produce antibody.