摘要
以摇瓶发酵为基础,在尽量提高酿酒酵母菌生物量的前提下,采用单因素与响应曲面相结合的设计方法,对影响优选酿酒酵母菌(Saccharomyces cerevisia)LFN518生物量的培养基相关的主要成分进行了优化,然后建立数学模型,通过响应曲面分析得到最优的酵母培养基配方为麦芽糖31.6g/L,酵母浸粉18.1g/L,磷酸氢二钾8.0g/L,硫酸镁12.0g/L,氯化钙6.0g/L,维生素母液6.0mL/L,酿酒酵母的生物量为8.124g/L,比优化前的6.25g/L提高了29.984%。
Based on shake flask results and in order to improve the biomass as much as possible, the main components in the medium, which affect the biomass of Saccharomyces cerevisia LFN518, were optimized by the combination of single factor experiment and response surface methodology. The related mathematical model was eastablished. The optimal medium was obtained by response surface analysis as maltose 31.6 g/L, yeast extract 18.1 g/L, potassium hydrogen phosphate 8.0 g/L, magnesium sulfate 12.0 g/L, calcium chloride 6.0 g/L and vitamin mother liquor 6.0 ml/L. The biomass of Saccharomyces cerevisiae grew on optimal medium was 8.124 g/L, which was 29.984% higher than that before optimization （6,25 g/L）.
出处
《中国酿造》
CAS
北大核心
2010年第8期18-21,共4页
China Brewing
基金
国家葡萄产业技术体系建设专项经费(nycytx-30)
