海洋弧菌Vibrio sp.pro1产碱性蛋白酶发酵条件的研究

Fermentation conditions forproduction of alkaline protease by marine bacterium Vibrio sp. pro1

对一株产碱性蛋白酶海洋弧菌(Vibrio sp.pro1)的发酵条件进行了单因素和正交试验,研究了不同碳源、无机氮源、pH值、温度、装液量、接种量等对产酶的影响。结果表明,液体培养基最佳组成为蛋白胨5.0g/L,酵母膏1.0g/L,硝酸钾0.6g/L,氯化镁4.0g/L。摇瓶培养的最佳条件为初始pH值为8.5,发酵温度33℃,装液量40.0mL,接种量2.0mL,优化后产酶量提高了6.3倍。

Fermentation conditions of a marine bacterium strain Vibrio sp. pro 1, which produces alkaline protease, were optimized through single and orthogonal test. Effect of carbon sources, inorganic nitrogen sources, pH, temperature, filling volume, inoculum were studied. It is showed that the optimum broth was consisted of 5 g/l peptone, 1.0 g/1 yeast extract, 0.6 g/1 KNO3 and 4.0 g/1 MgC12. The optimum fermentation conditions were as follows： filling volume at 40 ml, with initial pH 8.5 and inoculum at 2 ml, fermentation temperature under 33℃. Under the optimum conditions, yield of alkalase was 6.3 times higher than that of under original conditions.