鹌鹑H9亚型禽流感病毒的分离鉴定

Isolation and Identification of H9 Subtype Avian Influenza Virus from Quails

2009年从湖北省某鹌鹑(Coturnix coturnix Linnaeus)饲养场送检的成年产蛋病死鹌鹑的肺、脑等实质器官中,分离到一株低致病力的禽流感病毒(Avian influenza virus,AIV),经AIV-PCR检测、血清中和试验、AIVHA-HI试验、ND-HI试验,证明分离的AIV为H9亚型。人工感染30日龄鹌鹑做毒力测定,结果为低致病力毒株,该毒株自然感染能引起1%～3%的成年鹌鹑死亡。

An Avian influenza virus （AIV） strain was isolated from the brain and lung of quails（Coturnix coturnix Linnaeus） in 2009 at Hubei province.It was identified as H9 subtype by PCR, serum neutralization and hemagg lutination inhibition tests, and was further demonstrated to be avirulent by experimental infection of 30-day-old quails.The AIV strain could lead to 1% to 3% lethality of adult quails after natural infection.