扇子花(Scaevola nitida)的组织培养直接再生研究

Researches on Direct Regeneration in Vitro of Scaevola nitida

以扇子花(Scaevola nitida R.Br)的叶片为外植体,研究了其组培直接再生技术。试验结果表明,外植体消毒以0.1%HgCl2处理10min为佳;诱导叶片直接再生不定芽的合适培养基为MS+1.5mg/L6-BA;最佳壮苗培养基为MS+3%蔗糖,其中有机养分的含量为MS基本培养基的1.5倍。当培养基为1/2MS+3%蔗糖+0.1mg/L6-BA+0.3mg/LNAA,并以珍珠岩代替琼脂时,生根率可达92.22%,平均生根数6.02条,平均根长3.88cm,根较粗壮。

Leaves were used as explants to research the direct organogenesis via tissue culture for fan flower （Scaevola nitida R.Br.）.The results showed that the best treatment was to sterilize the explants with 0.1% HgCl2 for 10 min; MS＋1.5 mg/ L 6-BA＋0.01 mg/ L NAA and MS＋1.5 mg/ L 6-BA were the suitable media for direct shoots induction from leaves ; The concentration of sucrose and organic nutrients had extremely significant effect of shoots growth.MS with 3% sucrose and organic nutrients of 1.5×MS could make the plant stronger; good rooting results could be obtained with 1/2MS＋3% sucrose＋ 0.1 mg/L 6-BA＋0.3 mg/L NAA and replacing agar with perlite, which rooting percentage was 92.22%, average roots number was 6.02, average roots length was 3.88 cm and the roots were strong.