^(125)碘标记蛋白A及其在IgG类抗体免疫复合物检测中的应用

APPLICATIONS OF ^(125)I-LABELLED PROTEIN A IN DETECTING IgG IMMUNE COMPLEXES

本文对SPA的放射性标记、分离、储存及反应规律进行了实验研究,并成功地利用^(125)I-SPA 作为一种放射性示踪剂在国内首次相继建立了甲状腺刺激性抗体放射受体分析、旋毛虫抗体固相放射免疫分析、特异性 IgG 循环免疫复合物放射免疫分析等三种方法。研究结果表明,^(125)I-SPA 在以上三种方法中均获得满意的期望结果。因此,本文进一步推荐了一种特异性 IgG类抗体检测的通用程序。 作者认为:^(125)I-SPA 作为一种特异、灵敏、通用性强的放射性示踪配体,在免疫学实验方法研究中具有很高的实用价值。

Several laboratory applications of 125I-SPA, including the radio-receptor assay of TSabs, the solidphase RIA of trichinosis antibodies and the RIA of IgG-CIC, have been evolved in the present research. All methods are based on the selective binding of SPA to the Fc region of IgG. A general procedure was recommended for detection of IgG antibodies These applications of 125I-SPA show that being a specific, sensitive and general radio-tracing reagent. SPA can be used in a variety of immunological techniques.