摘要
根据NCBI中生长素受体蛋白TIR1基因上游1.5kb序列设计引物,以超级杂交稻株1S基因组DNA为模板,PCR扩增得到TIR1基因的启动子片段。将该片段克隆到pMD19-T载体后进行测序,获得长度为1530bp的序列。用PLACE软件分析发现该序列不仅具有启动子的基本元件TATA-box、CAAT-box,并具有多个胁迫响应元件,如光诱导元件、热诱导元件、生长素响应元件等。将该启动子与GUS基因融合,构建成表达载体后,可用于转化植物,为进一步研究水稻生长素受体蛋白TIR1基因的表达调控奠定了基础。
Primers were designed according to the 1.5kb upstream sequence of auxin-receptor TIR1 gene in NCBI database, and the promoter of TIR1 was obtained by PCR from genomic DNA of super hybrid rice Zhu 1S. The sequencing result showed that the promoter fragment was of length 1530bp. With the PLACE software analysis, it revealed that there were not only basic components （TATA box and CAAT box）, but also several stress response components （light responsive element, heat responsive element, auxin responsive element） in the promoter fragment. The expression vector, promoter fuse with GUS gene, could be used for plant transformation, and it would set up a solid foundation for further research on the mechanism of expression and regulation of TIR1 in rice.
出处
《湖南农业科学》
2010年第7期1-3,6,共4页
Hunan Agricultural Sciences
基金
湖南省教育厅资助科研项目(09K05
09C503)
