摘要
建立了检测ＥＤＳ７６病毒抗体Ｄｏｔ－ＥＬＩＳＡ方法。待检血清最适稀释度为１∶１０，ＥＤＳ７６病毒ＤＥＦ细胞培养物最佳工作浓度为１∶５，ＨＲＰ－ＭｃＡｂ工作浓度为１∶４００。所建方法与常见几种鸡的传染病血清无交叉反应，１２０份临床样本中检出６６份阳性。
A Dot ELISA to detect antibodies to EDS 76 virus has been developed The optimal dilutions of sera,DEF cell culture of EDS 76 virus and HRP McAb were 1∶10、1∶5 and 1∶40 respectively 120 sera were detected resuting in 66 positive reactions
出处
《中国动物检疫》
CAS
1999年第2期1-2,共2页
China Animal Health Inspection
