扁平苔癣免疫荧光检测的临床意义

IMMUNOFLUORESCENCE EXAMINATION ON LICHEN PLANUS AND ITS CLINICAL SIGNIFICANCE

本文对 13例皮肤 LP和 10例口腔粘膜 LP作 DIF检查,在真皮上部或粘膜下发现细胞样体染色,阳性率分别为77％和60％,有助于本病的诊断。进一步对皮肤或口腔粘膜LP各 8例和皮肤淀粉样变 3例的标本,用单抗 AE_1 和 AE_3 作 IIF检查,DIF 示细胞样体阳性的 LP标本全部被单抗 AE_1 或AE_3 染色。相反,阳性染色的皮肤淀粉样变和细胞样体阴性的 LP标本不能被上述单抗着染。本文就此结果的临床意义及发病机理进行讨论。

Immunopathological examinations of biopsies by direct immunofluorescence stain-ing (DIF) were done in 13 cases of cutaneous and 10 cases of oral mucosal lichen plan-us (LP). Positive cytoid body staining was found in 77% and 60% of the cutaneous and oral cases respectively. Indirect immunofluorescence staining (IIF) using monoclonal antibodies AE1 and AE3 were performed in 8 cases of cutaneous type as well as 8 cases of oral mucosal type. Those with positive DIF were all stainable with AE, and AE,. However, in 3 DIF positive biopsies of cutaneous amyloidosis used as controls, IIF were negative. The mechanism and clinical significance were discussed.