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表达结核杆菌ESAT-6基因重组耻垢分枝杆菌的免疫功能研究 被引量：3

Study on the Immunological Effects of Mycobacterium Smegmatis Expressing ESAT-6 in Mice
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摘要 目的研究表达结核杆菌ESAT-6基因重组耻垢分枝杆菌在动物体内的免疫功能。方法用表达结核杆菌ESAT-6基因重组耻垢分枝杆菌免疫BALB/c小鼠,通过淋巴细胞增殖实验检测重组耻垢分枝杆菌的免疫原性,利用流式细胞术分析小鼠T淋巴细胞亚群比例的变化,通过病理学切片观察重组耻垢分枝杆菌在动物体内的毒力。结果重组耻垢分枝杆菌诱导的脾淋巴细胞增殖反应、CD4+T细胞比例均明显高于对照组;重组耻垢分枝杆菌不会导致脏器的器质性病变。结论表达结核杆菌ESAT-6基因重组耻垢分枝杆菌免疫原性显著增强,且毒力无明显改变,有望成为一种新型的结核病疫苗。 Objective To investigate the immunological effects of mycobacterium smegmatis expressing ESAT-6 in mice.Methods BALB/c mice were immunized by mycobacterium smegmatis expressing ESAT-6.The proliferative response of spleen lymphocyte were assayed,the CD4＋ /CD8＋ cell proportion was detected by flow cytometry.Results The parameters in mycobacterium smegmatis expressing ESAT-6 immunized mice were significantly higher than those in the control group mice,suggesting that mycobacterium smegmatis expressing ESAT-6 possessed a great immunogenicity.Organ histology had no evidence pathological changes which indicated that the virulence of recombinant M.smegmatis was not enhanced and could be safely used for further study.Conclusion The recombinant M.smegmatis expressing ESAT-6 prossess immunogenicity in vivo and can be used as a novel vaccine against tuberculosis.
作者 李岩 鲍朗 李娅莎
机构地区 广州中医药大学基础医学院 四川大学华西医学中心基础与法医学院
出处 《临床医学工程》 2010年第8期15-17,共3页 Clinical Medicine & Engineering
基金 广东省高校重点培养青年教师基金(编号:K5090005)
关键词 ESAT-6 小鼠 耻垢分枝杆菌 ESAT-6 Mice Mycobacterium smegmatis
分类号 R378.911 [医药卫生—病原生物学]
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参考文献9

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二级参考文献9

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临床医学工程
2010年 第8期

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