自发高血压大鼠左室肥厚心肌Kv4.2表达的动态变化

The Dynamic Change of Kv4.2 Expression in Left Ventricular Hypertrophied Myocardium of Spontaneously Hypertensive Rat

目的探讨自发高血压大鼠左室肥厚心肌Kv4.2表达的动态演变规律。方法采用Western-Blot方法分别测定10、20和30周龄自发高血压大鼠左室心肌Kv4.2,同时测定大鼠心脏质量指数和左心室质量指数,以10周龄Wistar-Kyoto大鼠为对照组。结果①自发高血压大鼠的左心室质量指数和心脏质量指数明显大于Wistar-Kyoto大鼠(P<0.01);在自发高血压大鼠中,各组大鼠的心脏质量指数和左心室质量指数具有明显差别(P<0.01)。②自发高血压大鼠左室肥厚心肌Kv4.2的表达明显低于Wistar-Kyoto大鼠(P<0.01);在自发高血压大鼠中,各组大鼠左室肥厚心肌Kv4.2的表达具有明显差别(P<0.01)。③Kv4.2的表达与左心室质量指数呈负相关关系(r=-0.99,P<0.01)。结论左心室肥厚越明显,心肌Kv4.2的表达越低。

Objective To study the dynamic change of Kv4.2 expression in left ventricular hypertrophied myocardium of sponta-neously hypertensive rat（SHR）.Methods Kv4.2 expression was measured in left ventricular myocardium of 10,20 and 30-week SHR by the Western-Blot technique,at the same time,the heart mass index（HMI） and left ventricular mass index（LVMI） were also studied.The 10-week Wistar-Kyoto rat was served as the control group.Results ①The LVMI and HMI of SHR were significantly higher than those of Wistar-Kyoto rat（P0.01）.In SHR,the LVMI and HMI were significantly different（P0.01）.②The Kv4.2 expression in SHR was signifi cantly lower than that of Wistar-Kyoto rat（P0.01）.In SHR,the Kv4.2 expression was significantly different（P0.01）.③There was significantly negative relationship between the Kv4.2 expression and LVMI（r=-0.99,P0.01）.Conclusion The more significant left ventricular hypertrophy,the lower myocardial Kv4.2 expression.