髓细胞白血病患者AMLR及单核细胞Ia抗原表达的改变

THE CHANGES OF AMLR AND THE EXPRESSION OFHLA-II ANTIGENS ON MONOCYTES IN PATIENTSWITH MYELOID LEUKEMIA

T细胞在自身非T细胞刺激下发生增殖反应称为自身混合淋巴细胞反应(AMLR)。它反映机体免疫系统内的功能调节机制。本文以氚标胸腺嘧啶(~3H-TdR)掺入的淋巴细胞转化试验测定单核细胞(Mon)刺激自身T细胞的增殖反应;用单克隆抗体(McAb)Tu22、Tu36和 Anti-Leu-M,通过间接免疫荧光技术测定 Mon表面 HLA-Ⅱ类抗原的表达,分析急、慢性髓细胞白血病(AML 和CML)患者 AMLR 的改变及其机理。结果表明,与 HLA 全相同的同胞比较,患者 AMLR 明显减弱(p<0.001);患者Tu22^+(HLA-DQ^+)Mon 百分率明显下降(p<0.001),但Tu36^+(HLA-DR^+)Mon 百分率无明显改变(P>0.05)。提示髓细胞白血病患者 AMLR改变与刺激细胞(Mon)表面HLA-DQ 抗原表达障碍有关。

The autologous mixed lymphocyte reaction was measured with isotope incorporation technic and expression of HLA class II antigens on peripheral blood inonocytes was analyzed by indirect immunofluorescence assay with monoclonal antibodies Tu22, Tu36 and Anti-Leu-M2 in patients with acute and chronic myeloid leukemia (AML, CML). All patients (17 AML and 13 CML) demonstrated impaired autologous mixed lymphocyte reaction (p<0.001) as well as a markedly decreased proportion of MAC-120- and HLA-DQ-positive monocytes as compared with controls (p<0.001), while the percentage of HLA-DR-positive monocytes in the patients was unchanged (p>0.05). The controls in these studies were the patients' HLA identical normal siblings exclusively. The results suggested that impaired autologous mixed lymphocyte reaction was correlated with the decreased expression of HLA-DQ antigen on monocytes in patients with acute and chronic myeloid leukemia.