摘要
目的：建立血浆茶碱浓度监测的高效液相色谱方法。方法：血浆经高氯酸沉淀，以ZORBAXEclipseXDB--C18为色谱柱；流动相为甲醇-水-0．1％TFA体系，流速为0．8mL·min^-1；检测波长为274nm。结果：茶碱浓度在0．25～40．00mg·L^-1范围内线性关系良好（r=0．9999）；定量下限为0．25mg·L^-1；高中低三个浓度的相对回收率分别为（98．65±2．24）％、（101．62±1．85）％和（100．92±1．31）％。对54例患者做茶碱血药监测，临床效果满意。结论：本方法准确可靠、简便快速，可用于茶碱血药浓度监测的常规方法。
Objective To develop a high performance liquid chromatography method for the determination of theophlline in plasma. METHODS The plasma were precipitated by perchloric acid. The analytical column was packed with ZORBAX Eclipse XDB--C18. The mobile phase were methanol-- water--0.1% trifluoroacetic acid and the flow rate was 0.8 mL · min^-1. The UV detection wavelength was 274 nm. RESULTS Excellent liner relationship was obtained from the range of 0.25 mg · L^-1 to 40.00 mg · L^-1（r=0. 999 9）, the limit determination of doxifluridine was 0.25 mg · L^-1. The relative recoveries were （98.65±2.24） %, （101.62±1.85） %and （100. 92±1.31） % respectively at three concentrations. The clinical application was good. CONCLUSION The method was accurate, simple, rapid and could be used to TDM as a roution method.
