摘要
目的改进正常子宫内膜和子宫内膜异位症在位和异位内膜间质细胞原代和传代培养的方法 ,以使其可作为的体外实验模型之一。方法在原代分离细胞、传代培养条件和细胞纯化等方面对子宫内膜间质细胞原代培养的方法作了改进。结果 43例标本培养成功37例,仅1例污染,角蛋白、波形蛋白染色证实细胞纯度达95%以上符合实验要求。结论改进后的子宫内膜间质细胞原代培养法克服了污染问题,增加了可获得的细胞数,子宫内膜间质细胞的分离、培养可作为子宫内膜异位症的体外实验模型之一。
Objective To improve the primary cell and passage cell culture methods of endometrial stromal cells,which would be one of the in vitro models of endometriosis.Methods Improved the procedure of separated primary cell,adjusted passage cell culture conditions and ESC purification.Results Of the 43 specimens,37 were successfully cultured and only 1 contaminated.The purity of stromal cells was 95% identified by cytokeratin and vimentin staining.Conclusion The yields of stromal cells could be significantly increased by modification of the procedure contamination.The isolation and culture of endometrial stromal epithelial cells can be one of the in vitro models of endometriosis.
出处
《慢性病学杂志》
2010年第8期846-847,849,共3页
Chronic Pathematology Journal
