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经顺铂诱导的PC3-TR细胞对肿瘤因子相关凋亡诱导配体敏感性的影响

The effect of DDP enhancing the sensitivity of TRAIL inhibiting PC3-TR
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摘要 目的探讨经顺铂(DDP)诱导的PC3-TR细胞对肿瘤坏死因子相关凋亡诱导配体(TRAIL)敏感性的影响。方法将PC3-TR细胞分为3组。DDP组以1.0、5.0、10.0、20.0、50.0、100.0μg/ml浓度、TRAIL组以10.0、20.0、50.0、100.0ng/ml浓度、DDP5.0或10μg/ml+TRAIL50.0ng/ml处理PC3-TR细胞,24h后MTT法检测细胞的吸光度;DDP+TRIL组以按细胞抑制率(IR)=(1-实验组吸光度/对照组吸光度)×100%计算各组对细胞的IR,比较组间细胞的IR。结果单独应用不同浓度DDP及TRAIL对PC3-TR细胞抑制作用不明显(P>0.05),小剂量DDP(5.0μg/ml及10.0μg/ml)与0.5ng/mlTRAIL联合处理PC3-TR细胞,对PC3-TR细胞有明显的抑制效应,与相应浓度的DDP、TRAIL单独应用组及空白对照组比较,差异有统计学意义(P<0.01)。结论 DDP能诱导PC3-TR细胞株DR5受体的水平提高,增强了PC3-TR细胞对TRAIL的敏感性,逆转PC3-TR细胞株对TRAIL的耐受,明显提高TRAIL对前列腺癌PC3-TR细胞株的抑制效应。 Objective To investigate the experiment that DDP induced DR5 receptor and enhanced the sensitivity of TRAIL-inhibiting PC3-TR.Methods The PC3-TR cells were separately treated with different concentrations of DDP（1.0,5.0,10.0,20.0,50.0,100.0μg/ml）,TRAIL（10.0,20.0,50.0,100.0,200.0ng/ml）,DDP（5.0μg/ml or 10μg/ml）in combination with TRAIL（50.0ng/ml）.And 24h later cell absorbance was detected by MTT assay.The inhibitory rate of cells was calculated with the inhibitory rate of cells（IR）=（1-absorbance of experimental group/absorbance of control group）×100%.Compared with IR of different groups.Results DDP and TRAIL treated with PC3-TR separately and the effect of inhibiting PC3-TR was not obvious（P0.05）.Small doses of DDP had the obvious effect of inhibiting PC3-TR in combination with TRAIL,and compared with the corresponding groups of DDP and TRAIL separately,（P0.01）.Conclusion The level of DDP inducing DR5 increased and DDP could enhance the sensitivity of TRAIL inhibiting PC3-TR.The state of TRAIL to lerated PC3-TR was reversed.DDP can significantly enhance the effect of TRAIL inhibiting PC3-TR.
作者 曾四平 章小平 肖亚军 钱晓辉 李炎生
机构地区 广西医科大学第四附属医院柳州市工人医院 华中科技大学同济医学院附属协和医院
出处 《临床合理用药杂志》 2010年第16期50-52,共3页 Chinese Journal of Clinical Rational Drug Use
基金 国家自然科学基金项目(No:30572139)
关键词 肿瘤因子相关凋亡诱导配体 顺铂 DR5 TRAIL DDP Death receptor5（DR5）
分类号 R730.5 [医药卫生—肿瘤]
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参考文献8

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二级参考文献15

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临床合理用药杂志
2010年 第16期

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