摘要
目的建立测定咽炎颗粒中蒙花苷含量的高效液相色谱法。方法采用高效液相色谱法。色谱柱:CAPCEll PAKC18(5μm,250mm×4.6mm);流动相:甲醇-1%醋酸溶液(49∶51);检测波长:334nm;流速:1.0ml/min;柱温:40℃。结果蒙花苷在4.64～69.6μg范围内呈良好的线性关系(r=0.9999)。蒙花苷的平均回收率为99.33%,RSD为0.79%(n=6)。结论该方法准确、灵敏度高,重复性好。
Objective To establish a high performance liquid chromatography（HPLC）method for determination of buddleoside in Yanyan Granule.Methods CAPCEll PAK C18（5μm,250mm×4.6mm）was used.methanol-1%glacial（49：51）as the mobile phase,with detection wavelength at 220nm and the flow rate was 1.0ml/min.Results Buddleoside had a good linear relationship in the range of 4.46~69.6μg （r=0.9999）.The average recovery of buddleoside was 99.33%,RSD was 0.79%（n=6）.Conclusion The method is reliable,accurate and reproducibble.
出处
《临床合理用药杂志》
2010年第16期61-62,共2页
Chinese Journal of Clinical Rational Drug Use
