寒兰品种类型的SRAP分子鉴定

Analysis on Cymbidium kanran with SRAP Markers

【目的】从分子水平分析寒兰品种的遗传多样性和亲缘关系,为寒兰种质资源的有效利用和开发提供依据。【方法】利用SRAP标记对37个中国寒兰和14个日本寒兰品种类型的基因组DNA进行分析。【结果】筛选出的11对引物组合对51份供试材料共扩增出586条带纹,其中504条为多态性(86.01%),平均每个引物扩增46条多态性带。聚类分析表明,51份材料根据起源和生物学特性划分为中国寒兰和日本寒兰两大类群。【结论】SRAP标记技术能很好地用于兰花植物遗传多样性和亲缘关系研究。

【Objective】 The genetic diversity and relationships of Cymbidium kanran resources were studied at molecular level for providing new molecular evidences of utilization and exploitation of germplasm resources of Cymbidium kanran. 【Method】 The sequence-related amplified polymorphism （SRAP） was assessed to analyze the genetic relationships among 51 Cymbidium kanran cultivars, thirty-seven of them originated from China, others from Japan. 【Result】 A total of 586 DNA bands were amplified by 11 selective primers, 504 of which （86.01%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 46. Cluster analysis showed that the cymbidium could be divided into two clusters （Chinese orchid and Japanese orchid） based on geographic and ecological traits. 【Conclusion】 It was concluded that the SRAP technique has provided a powerful tool for cultivar identification and establishment of genetic relationships of cultivars in Cymbidium kanran.