摘要
以近200个巴西橡胶树种质材料(品种及无性系)幼嫩叶片为材料,采用改良的CTAB法对其基因组总DNA的提取进行了研究。结果表明,该方法提取的DNA溶液无色透明,OD260/OD280的比值均为1.8～2.0,1%琼脂糖凝胶电泳为清晰的一条带,无降解现象,RNA去除干净,能被限制性内切酶完全消化;其AFLP分析(引物EcoRI-TA/MseI-CTT)条带清晰,多态性好,说明此方法提取的巴西橡胶树幼嫩叶片基因组总DNA较适于AFLP分析。
The genomic DNA samples of tender leaves of approximately two hundred germplasms of Hevea brasiliensis Muell. Arg. （varieties and clones） were isolated by improved CTAB method. The results showed that the extracted DNA of mature leaves by this method was pure and integral. The value of OD260/OD280 was 1.8 to 2.0. Degradation was nearly free. The RNA was eliminated completely, and suitable for digestion by restriction endonucleases. AFLP analysis indicated the primer （EcoRI-TA/MseI- CTT） produced clear polymorphic patterns. Therefore this method could be used for extracting ideal DNA samples from tender leaves of Hevea brasiliensis Muell. Arg. for AFLP analysis.
出处
《热带农业科学》
2010年第5期1-5,共5页
Chinese Journal of Tropical Agriculture
基金
"十一五"国家科技支撑计划(No.2006BAD01A06-3)
植物新品种DUS测试指南研制项目
关键词
巴西橡胶树
AFLP分析
DNA提取
Hevea brasiliensis Muell Arg.
AFLP analysis
DNA extraction
