微波辐照对感光细胞蛋白结构影响的光谱分析

Spectroscopic analysis on rat photoreceptor proteins structure after microwave irradiation

研究高功率微波(HPM)照射后感光细胞中膜蛋白分子结构的改变,以探讨HPM的损伤机制。选取大鼠视网膜感光细胞外节层作为研究对象,通过对FT-IR图谱酰胺Ⅰ带中蛋白质二级结构的各吸收峰进行定量分析,观察HPM照后蛋白质分子结构的改变情况。结果显示HPM照后外节出现了膜蛋白二级结构吸收峰的位移和吸收值的改变。该研究表明HPM照射可使视网膜感光细胞的膜蛋白质构象发生改变,导致蛋白质结构稳定性下降和蛋白生物功能的破坏。

To study the bioeffects of HPM on animal photoreceptor proteins structure and explore the HPM injury molecular mechanism,FT-IR microspectroscopy is applied to observe the change of biomembranes proteins structure in the outer segments.The results by FT-IR reveals that there are significant spectral differences of the absorption values for amideⅠband and the α-helix conformation decreases along with the increase of random coil and β-sheets structures after HPM exposure.It comes to the conclusion that the secondary structure of retinal membrane proteins has changed since the HPM irradiation,which may lead to the loss of proteins function.