组胺H_3受体拮抗剂对吗啡成瘾大鼠复吸行为的影响

Effect of histamine H_3 receptor antagonist on morphine-dependent rats in relapse behavior

目的研究不同剂量的组胺H3受体拮抗剂在吗啡成瘾大鼠复吸行为中的影响。方法将将大鼠分成4组,组胺H3受体拮抗剂低剂量组、中剂量组、高剂量组、生理盐水对照组。以吗啡剂量递增法、自然戒断和药物点燃等方法复制以上各组大鼠模型,用条件性位置偏爱法判断复制模型成功与否。结果组胺H3受体拮抗剂低剂量组在注射拮抗剂(5mg/kg)后经吗啡点燃,表现出明显偏爱于白箱(P<0.01,与黑箱比较);组胺H3受体拮抗剂中剂量组在注射拮抗剂(10mg/kg)后经吗啡点燃,表现出明显偏爱于白箱(P<0.01,与黑箱比较);组胺H3受体拮抗剂高剂量组在注射拮抗剂(20mg/kg)后经吗啡点燃后,无明显偏爱表现(P>0.05,与黑箱比较);生理盐水对照组在注射生理盐水后经吗啡点燃后,表现出明显偏爱于白箱(P<0.01与黑箱比较)。结论低剂量和中剂量组胺H3受体拮抗剂在吗啡成瘾大鼠复吸中无明显作用。高剂量组胺H3受体拮抗剂在吗啡成瘾大鼠复吸中有明显作用。

Objective To study the influence of different doses of histamine H3 receptor antagonist on morphine addicted rats relapse behavior.Methods The rats were divided into 4 groups： histamine H3 receptor antagonist with low dose group,middle dose group and high dose group,saline control group.To gradually increasing doses of morphine,natural light and other methods of drug withdrawal and copy the above groups,rats,conditioned place preference method used to judge the success of replication models.Results Histamine H3 receptor antagonist injection of antagonist in the low dose group（5mg/ kg） by morphine after the fire,showing an obvious preference for the white box（P0.01,compared with the black box）;histamine H3 receptor antagonist in the dose antagonist in the injection group（10mg/kg） of morphine after the fire by,showing clear preference in the white box（P0.01,compared with the black box）;histamine H3 receptor antagonist with high dose injection of antagonist（20mg/kg） then ignited after morphine,no significant preference for performance（P 0.05,compared with the black box）;saline control group after saline injection by morphine lit display an obvious preference for the white box（P0.01 compared with the black box）.Conclusions Low dose and middle dose histamine H3 receptor antagonist in morphine addicted rats addiction recurrence no effect.High dose histamine H3 receptor antagonist in morphine addicted rats readdicted have obvious role.