VA菌根真菌对转移Ri T-DNA胡萝卜根器官的侵染

INFECTION OF TRANSFORMED Ri T DNA CARROT ROOTS BY GIGASPORA MARGARITA

在无菌条件下观察珠状巨孢囊霉（Ｇｉｇａｓｐｏｒａｍａｒｇａｒｉｔａ）对转移ＲｉＴＤＮＡ胡萝卜根器官的侵染过程。采用发根土壤杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）诱导出细胞中整合有ＲｉＴＤＮＡ胡萝卜的不定根作宿主，比较了孢子的４种表面消毒方法，经过２～３个月的双重培养，看到ＶＡ菌根真菌对根器官的侵染，并获得成熟孢子。形成的孢子无须休眠，只要提供发芽条件，就能立即发芽，接种到根的周围又能侵染。文中对侵染的全过程作了较详细的描述。

This paper described the infection process of vesicular arbuscular mycorrhizal fungus Gigaspora margarita in transformed Ri T DNA carrot roots under axenic condition Agrobacterium rhizogenes was used to introduce Ri T DNA into the root cells of carrot and then the adventitious roots of carrot were used as host Four routine methods of surface sterilization were compared and one was selected for obtaining maximum amount of pure mycorrhizal fungal spores After two months of dual culture, the infection of transformed Ri T DNA carrot roots by VA mycorrhizal fungus was observed and mature spores were obtained Under suitable conditions, the harvested spores were capable of germinating and subsequently infecting the host roots immediately