OXA-23基因阳性耐亚胺培南鲍曼不动杆菌感染暴发调查研究

An infection outbreak of OXA-23 carbapenemase-producing imipenem-re-sistant Acinetobacter haumannii

目的了解某院神经内科重症监护室(ICU)暴发流行耐亚胺培南鲍曼不动杆菌感染的基因型及耐药机制。方法以API20NE鉴定菌株,纸片扩散法测定分离菌株对常用抗菌药物的敏感性,聚合酶链反应(PCR)扩增3种酶基因(OXA、IMP、VIM),并对产物进行测序。结果患者痰标本分离的7株耐亚胺培南鲍曼不动杆菌均携带OXA-23基因,未检出IMP、VIM基因。结论此次暴发流行中,耐碳青霉烯类药物的鲍曼不动杆菌携带的耐药基因为OXA-23基因。

Objective To evaluate the gene types and drug resistance mechanism of imipenem-resistant Acineto- bacterbaurnannii （A. baumannii） causing infection outbreak in an intensive care unit （ICU） . Methods A. baumannii isolates were identified by API20 NE, antimicrobial susceptibility were detected by disc agar diffusion methods . Three genes including OXA, IMP, and VIM were amplified by polymerase chain reaction （PCR）, sequences were analysed using BLAST 2. 0. Results Seven imipenem-resistant A. baurnannii isolates all carried OXA-23 gene, IMP and VIM genes were not detected. Conclusion The outbreak was mainly caused by A. baumannii which carried OXA-23 gene.