卒中患者瘦素水平及瘦素受体基因多态性的实验研究

Serum leptin level and polymorphism of leptin receptor gene in patients with stroke

目的 探讨血清瘦素水平及瘦素受体基因多态性与卒中发病的关系. 方法 选择广州红十字会医院神经内科自2007年1月至2008年12月99例住院卒中患者[脑血管病（CVD）组]及97例同期住院的非卒中患者（对照组）做为研究对象,采用聚合酶链反应结合限制性片段长度多态性对2组患者进行瘦素受体基因Gln223Arg多态性进行检测,ELISA分析法检测血清瘦素水平. 结果 CVD组患者瘦素受体Gln223Arg的GG、GA、AA基因型分布频率分别为66.67%、20.20%、13.13%,与对照组的78.35%、15.46%、6.19%分布频率差异无统计学意义（P＞0.05）;CVD组G和A等位基因频率分别为76.77%和25.23%,与对照组的86.08%和13.92%基因分布频率差异有统计学意义（P＜0.05）.CVD组的血清瘦素水平明显高于对照组,差异有统计学意义（P＜0.05）.非条件Logistic回归多因素分析表明高血压史、空腹血糖升高、TC升高、血清瘦素水平升高与卒中的发病相关. 结论 瘦素受体基因Gln223Arg的A等位基因、瘦素水平升高、高血压、空腹血糖升高、TC升高会增加卒中的发病风险.

Objective To study the relation between stroke and both plasma leptin level and polymorphism of leptin receptor gene. Methods Ninty-nine patients with stroke and 97 patients with other diseases （controls）, admitted to our hospital from January 2007 to December 2008, were chosen in the experiment. Polymerase chain reaction-restriction fragment length polymorphism （LEPR） examination was performed to detect the leptin receptor gene （Gln223A rg gene） polymorphism in these patients. The level of serum leptin was analyzed by enzyme linked immunosorbent assay. Results The genotype frequencies of GG, GA and AA in Gln223Arg gene in patients with stroke were 66.67%, 20.20% and 13.13%, respectively, while those in controls were 78.35%, 15.46% and 6.19%, respectively; those in these 2 groups were not significantly different （P＞0.05）. The allele frequencies of A and G in patients with stroke were 76.77% and 25.23%, while those in controls were 86.08% and 13.92%; those in the 2 groups were significantly different （P＜0.05）. The level ofleptin in patients with stroke was markedly higher that that in controls （P＜0.05）. Binary regression analyses showed that history of hypertension, elevated fasting blood glucose and cholesterol, increased plasma leptin level were associated with stroke. Conclusion The allele frequencies of A in Gln223A rg gene, hypertension, elevated fasting blood glucose and cholesterol and increased plasma leptin level might be correlated with the increased risk of stroke.